Patent 2017245395

Methods and reagents for creating monoclonal antibodies

Abstract

ABSTRACT In some embodiments, the invention relates to methods for creating a monoclonal antibody that specifically binds to antigen. The method may start from a polyclonal population of antibodies such as a non-specific polyclonal population or a polyclonal population of antibodies that specifically bind to the antigen. The method includes obtaining nucleic acid molecules encoding heavy and light immunoglobulin chains (or variable regions thereof) of multiple immunoglobulins from an animal; obtaining mass spectra information of peptide fragments of a population of polyclonal immunoglobulins that specifically bind to an antigen of choice; comparing and/or correlating the mass spectra information of the peptide fragments of the polyclonal immunoglobulins with predicted mass spectra information of predicted amino acid sequences encoded by the nucleic acid molecules, and then assembling the heavy and light chains to create an antibody (or variable region thereof) that specifically binds to the antigen.

Information

Australian application number
2017245395
Country
US
Status
Filed
Type
Standard
Applicant
Cell Signaling Technology, Inc., INC (US)
Application year
2017

International Patent Classification (IPC) codes

Links

ID Relationship
2012225310 Divisional parent